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KMID : 1101720190230010007
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Physical Activity and Nutrition
2019 Volume.23 No. 1 p.7 ~ p.12
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Effects of resveratrol on laminar shear stress-induced mitochondrial biogenesis in human vascular endothelial cells
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Kim Ji-Seok
Park Joon-Young
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Abstract
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Purpose: The purpose of the study was to determine the combined effects of resveratrol supplementation with high-flow LSS on mitochondrial biogenesis in human vascular endothelial cells.
Methods: Cultured human umbilical vein endothelial cells were treated with 20 μM of RSV. For the shear experiments, cells grown to a >90% confluence were exposed to physiological levels of LSS (5 to 20 dyne/cm2) for 12 to 36 hours using a cone and plate shear apparatus. Gene expressions were analyzed by western blotting.
Results: Depletion of mitochondrial integrity was directly associated with increase in endothelial activation/dysfunction. The expressions of mitochondrial biogenesis regulator genes, such as SIRT1, PGC-1α, and TFAM, and the mitochondrial contents were significantly increased after treatment with both resveratrol and high-flow LSS for 12 hours. However, supplementation of resveratrol to high-flow LSS for a prolonged duration had no synergistic effect on the levels of mitochondrial biogenesis regulator gene expressions and mitochondrial content compared to the LSS treatment alone.
Conclusion: The present study demonstrated that the supplementation of resveratrol to high-flow LSS has no synergistic effects on enhancing mitochondrial integrity in human vascular endothelial cells.
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KEYWORD
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Resveratrol, Laminar shear stress, SIRT1, Mitochondrial biogenesis
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